Effects of Vascular Endothelial Growth Factor on YAP/TAZ Signaling in Trabecular Meshwork Cells

Purpose: To investigate the effects of vascular endothelial growth factor (VEGF) on yes-associated protein (YAP)/transcriptional coactivator with a PDZ-binding motif (TAZ), Hippo pathway-related transcription factor, and role YAP/TAZ induced by trabecular meshwork stimulation.Methods: Human cells were cultured treated various VEGF concentrations to verify cell cytotoxicity using CCK-8 solution. Transforming β-2 (TGFβ2; 5 ng/mL) (30 applied expression was assessed western blotting, reverse quantitative polymerase chain reaction immunocytochemistry. Fibronectin, collagen 1, myocilin also blotting. The stained Alexa Fluor 488-phalloidin observe F-actin changes.Results: YAP TAZ increased following TGFβ2 treatment for 24 hours. Fibronectin 1 significantly in all three groups, while TGFβ2+VEGF groups. staining showed cross-linking cells.Conclusions: signaling fibrosis. Based functional changes caused VEGF, it is suggested that may increase aqueous humor outflow resistance cells.